Not known Factual Statements About flow cytometers
Not known Factual Statements About flow cytometers
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At the moment, the early signs had been dismissed as debris, however the retrospective evaluation shown shared attributes While using the problematic cells in culture. Tracing the infection aided the lab create extra laboratory strategies for screening and security of assay-important cell strains.
As samples are acquired on the Attune CytPix Flow Cytometer, the higher-velocity brightfield digital camera captures and merchants photographs of detected functions at a price as much as 6,000 photographs for each next, based on the flow fee and picture dimension. For bigger overall flexibility, Attune Cytometric Program lets you alter the image seize frequency as required and select a selected gate for graphic capture, if necessary.
(B) RAW 264.7 macrophages are often annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The number of singlets obtainable for Examination improves when the person trains and employs a more exact product.
Learn new insights into your experimental data by visualizing populations working with mixed fluorescence and morphology information. Our application interface wizards allow downsampling, concatenation (merged documents) and dimensionality equipment which can be designed in to the software for an intuitive, streamlined knowledge Evaluation working experience.
"A picture is just not rather value 1000 dots, nonetheless it definitely helps to see photos. It is great to check out visuals that correlate with staining. Such as, AnnexinV-good cells which are morphologically distinctive than healthy cells, or CD14+ monocytes which have been more substantial and much more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it is actually illuminating to check out what number of doublets slip by way of conventional singlet gates, and terrific to validate the standing of unforeseen double-good functions in my Examination."
Pair your Attune flow cytometer using an autosampler for increased workflow performance, and incorporate robotic integration for max throughput.
Consistent image excellent even at high throughput Acoustic concentrating and also a large-pace camera Merge to image these T cells persistently at low or higher flow rates. Conveniently alter emphasis and camera settings to satisfy experimental specifications.
The Attune NxT cytometer could be the trusted workhorse instrument your lab can count on for steady and trusted functionality. The Attune CytPix model incorporates a significant-velocity brightfield digital camera and AI-driven image Investigation software package, with modern updates which includes machine learning resources and an intuitive consumer interface, enabling Improved info precision, precision, and more rapidly insights into sample biology.
Probably most of all, these occasions incorporate cells of Evidently various phenotypes which may lead to incorrect conclusions with regards to double favourable events (especially in exceptional populations).
AI-pushed automatic picture Investigation While using the Attune Cytometric Program translates event features into unique morphology parameters which can be combined with normal fluorescence and scatter parameters to expose really reproducible inhabitants adjustments and one of a kind subsets.
Software Take note: Automatic image Assessment decreases consumer-to-consumer variability in flow cytometry gating techniques
Leverage the power of Innovative technology and innovation to accelerate your discoveries. Rely on within the Attune Flow Cytometer household to provide the dependability, pace, and accuracy that set your do the job aside.
No clean/no lyse leukocyte analysis with imaging. Cells have been acquired from 24-hour-outdated blood by dilution in one mM EDTA ( Expert UX/UI enables end users to practice AI products for expanded applications. (A) The “Train Product” element presents annotation tools and authentic-time model coaching accuracy.
At the heart of every Attune Flow Cytometer lies our innovative acoustic focusing know-how, which utilizes sound waves to meticulously align cells in the flow cell. This progressive solution surpasses regular hydrodynamic solutions, leading to more rapidly processing speeds, Excellent information quality, and enhanced sample throughput.
The Attune Xenith cytometer is often a superior-parameter here spectral analyzer, furnishing each spectral unmixing and conventional flow abilities for complete and adaptable cell analysis.
The Attune CytPix Flow Cytometer permits consumers to visualize samples through acquisition, verify the accuracy of gating approaches, and help results with dependable documentation for publication purposes.
What appeared to be a homogeneous population is definitely a lot more assorted—an insight that ought to be thought of when interpreting final results.